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addgene 52961 cropseq guide puro datlinger  (Addgene inc)


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    Structured Review

    Addgene inc addgene 52961 cropseq guide puro datlinger
    Addgene 52961 Cropseq Guide Puro Datlinger, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+52961+cropseq+guide+puro+datlinger/lentiCRISPR+v2+(Plasmid+%2352961)/pm39255790-549-165-165
    Average 96 stars, based on 6177 article reviews
    addgene 52961 cropseq guide puro datlinger - by Bioz Stars, 2026-10
    96/100 stars

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    Virus:

    Article Title: Pooled endogenous protein tagging and recruitment for systematic profiling of protein function.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Santa Cruz sc-9996; RRID: AB_627695 BRD4 Novus Biologicals NBP2-76393; RRID: AB_2631885 alpha Tubulin Novus Biologicals NB100-690; RRID: AB_521686 IRDye 680LT LI-COR 926–68021; RRID: AB_10706309 IRDye 800CW LI-COR 926–32210; RRID:AB_621842 Bacterial and virus strains Endura Electrocompetent Cells Lucigen 60242–2 One ShotTM Stbl3TM Chemically Competent E. coli Invitrogen C737303 ZYCY10P3S2T E. coli Minicircle producer strain System Biosciences MN900A-1 Experimental models: Cell lines HAP1 Horizon Discovery C859 HEK293, EGFP-FKBP Gift from Dr. Craig Crews29 (Yale University) N/A Critical commercial assays CellTiter 96 AQueous One Promega G3580 Chemicals, peptides, and recombinant proteins HaloFK-29 This paper N/A HaloFK-59 This paper N/A HaloFK-7 (i.e., PhosTAC79) MCE and gift from Dr. Craig Crews (Yale University) HY-145232 HaloBR-6 This paper N/A dTAGV-1 Tocris Bioscience 6914 JQ1 MCE HY-13030 dBET6 MCE HY-112588 HaloTag TMR Promega G8251 MG132 MilliporeSigma M7449 Recombinant DNA pMC_HT7_BSDminus_F0 This paper Addgene 225627 pMC_HT7_BSDminus_F1 This paper Addgene 225628 pMC_HT7_BSDminus_F2 This paper Addgene 225629 sgDonor_lentiGuide Serebrenik et al.36 Addgene 184168 lentiCRISPR v2 Sanjana et al.63 Addgene 52961 CROPseq-Guide-Puro Datlinger et al.64 Addgene 86708 Software and algorithms R v4.4.0 The R Project for Statistical Computing https://www.R-project.org/ ggplot2 v3.5.1 Wickham et al.65 https://ggplot2.tidyverse.org drc v3.0-1 Ritz et al.66 https://cran.r-project.org/package=drc CB2 v1.3.4 Jeong et al.67 https://doi.org/10.32614/CRAN.package.CB2 edgeR v3.32.1 Robinson et al.68 https://bioconductor.org/packages/release/bioc/ html/edgeR.html STRINGdb v2.16.4 Szklarczyk et al.69 https://www.bioconductor.org/packages/release/ bioc/html/STRINGdb.html gprofiler2 v0.2.3 Kolberg et al.70 https://doi.org/10.32614/CRAN.package.gprofiler2 e1 Cell Genomics 4, 100651, October 9, 2024 OPEN ACCESS .. Cell lines and culture details Cell lines, including HAP1 (Horizon Discovery) and HEK293T (ATCC CRL-3216) cells were handled according to manufacturer’s instructions.

    Recombinant:

    Article Title: Pooled endogenous protein tagging and recruitment for systematic profiling of protein function.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Santa Cruz sc-9996; RRID: AB_627695 BRD4 Novus Biologicals NBP2-76393; RRID: AB_2631885 alpha Tubulin Novus Biologicals NB100-690; RRID: AB_521686 IRDye 680LT LI-COR 926–68021; RRID: AB_10706309 IRDye 800CW LI-COR 926–32210; RRID:AB_621842 Bacterial and virus strains Endura Electrocompetent Cells Lucigen 60242–2 One ShotTM Stbl3TM Chemically Competent E. coli Invitrogen C737303 ZYCY10P3S2T E. coli Minicircle producer strain System Biosciences MN900A-1 Experimental models: Cell lines HAP1 Horizon Discovery C859 HEK293, EGFP-FKBP Gift from Dr. Craig Crews29 (Yale University) N/A Critical commercial assays CellTiter 96 AQueous One Promega G3580 Chemicals, peptides, and recombinant proteins HaloFK-29 This paper N/A HaloFK-59 This paper N/A HaloFK-7 (i.e., PhosTAC79) MCE and gift from Dr. Craig Crews (Yale University) HY-145232 HaloBR-6 This paper N/A dTAGV-1 Tocris Bioscience 6914 JQ1 MCE HY-13030 dBET6 MCE HY-112588 HaloTag TMR Promega G8251 MG132 MilliporeSigma M7449 Recombinant DNA pMC_HT7_BSDminus_F0 This paper Addgene 225627 pMC_HT7_BSDminus_F1 This paper Addgene 225628 pMC_HT7_BSDminus_F2 This paper Addgene 225629 sgDonor_lentiGuide Serebrenik et al.36 Addgene 184168 lentiCRISPR v2 Sanjana et al.63 Addgene 52961 CROPseq-Guide-Puro Datlinger et al.64 Addgene 86708 Software and algorithms R v4.4.0 The R Project for Statistical Computing https://www.R-project.org/ ggplot2 v3.5.1 Wickham et al.65 https://ggplot2.tidyverse.org drc v3.0-1 Ritz et al.66 https://cran.r-project.org/package=drc CB2 v1.3.4 Jeong et al.67 https://doi.org/10.32614/CRAN.package.CB2 edgeR v3.32.1 Robinson et al.68 https://bioconductor.org/packages/release/bioc/ html/edgeR.html STRINGdb v2.16.4 Szklarczyk et al.69 https://www.bioconductor.org/packages/release/ bioc/html/STRINGdb.html gprofiler2 v0.2.3 Kolberg et al.70 https://doi.org/10.32614/CRAN.package.gprofiler2 e1 Cell Genomics 4, 100651, October 9, 2024 OPEN ACCESS .. Cell lines and culture details Cell lines, including HAP1 (Horizon Discovery) and HEK293T (ATCC CRL-3216) cells were handled according to manufacturer’s instructions.

    Software:

    Article Title: Pooled endogenous protein tagging and recruitment for systematic profiling of protein function.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Santa Cruz sc-9996; RRID: AB_627695 BRD4 Novus Biologicals NBP2-76393; RRID: AB_2631885 alpha Tubulin Novus Biologicals NB100-690; RRID: AB_521686 IRDye 680LT LI-COR 926–68021; RRID: AB_10706309 IRDye 800CW LI-COR 926–32210; RRID:AB_621842 Bacterial and virus strains Endura Electrocompetent Cells Lucigen 60242–2 One ShotTM Stbl3TM Chemically Competent E. coli Invitrogen C737303 ZYCY10P3S2T E. coli Minicircle producer strain System Biosciences MN900A-1 Experimental models: Cell lines HAP1 Horizon Discovery C859 HEK293, EGFP-FKBP Gift from Dr. Craig Crews29 (Yale University) N/A Critical commercial assays CellTiter 96 AQueous One Promega G3580 Chemicals, peptides, and recombinant proteins HaloFK-29 This paper N/A HaloFK-59 This paper N/A HaloFK-7 (i.e., PhosTAC79) MCE and gift from Dr. Craig Crews (Yale University) HY-145232 HaloBR-6 This paper N/A dTAGV-1 Tocris Bioscience 6914 JQ1 MCE HY-13030 dBET6 MCE HY-112588 HaloTag TMR Promega G8251 MG132 MilliporeSigma M7449 Recombinant DNA pMC_HT7_BSDminus_F0 This paper Addgene 225627 pMC_HT7_BSDminus_F1 This paper Addgene 225628 pMC_HT7_BSDminus_F2 This paper Addgene 225629 sgDonor_lentiGuide Serebrenik et al.36 Addgene 184168 lentiCRISPR v2 Sanjana et al.63 Addgene 52961 CROPseq-Guide-Puro Datlinger et al.64 Addgene 86708 Software and algorithms R v4.4.0 The R Project for Statistical Computing https://www.R-project.org/ ggplot2 v3.5.1 Wickham et al.65 https://ggplot2.tidyverse.org drc v3.0-1 Ritz et al.66 https://cran.r-project.org/package=drc CB2 v1.3.4 Jeong et al.67 https://doi.org/10.32614/CRAN.package.CB2 edgeR v3.32.1 Robinson et al.68 https://bioconductor.org/packages/release/bioc/ html/edgeR.html STRINGdb v2.16.4 Szklarczyk et al.69 https://www.bioconductor.org/packages/release/ bioc/html/STRINGdb.html gprofiler2 v0.2.3 Kolberg et al.70 https://doi.org/10.32614/CRAN.package.gprofiler2 e1 Cell Genomics 4, 100651, October 9, 2024 OPEN ACCESS .. Cell lines and culture details Cell lines, including HAP1 (Horizon Discovery) and HEK293T (ATCC CRL-3216) cells were handled according to manufacturer’s instructions.



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    Addgene inc addgene 52961 cropseq guide puro datlinger
    Addgene 52961 Cropseq Guide Puro Datlinger, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+52961+cropseq+guide+puro+datlinger/lentiCRISPR+v2+(Plasmid+%2352961)/pm39255790-549-165-165
    Average 96 stars, based on 1 article reviews
    addgene 52961 cropseq guide puro datlinger - by Bioz Stars, 2026-10
    96/100 stars
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